| RORγt

RORγt (also known as RORγ2) – produced from an mRNA identical to that of RORγ, except that the two 5'-most exons are replaced by an alternative exon, located downstream in the gene. This causes a different, shorter N-terminus.RORγt, is expressed in various immune cells. Of those, the most prominent examples are immature CD4+/CD8+ thymocytes, T helper 17 (Th17) cells and in type 3 innate lymphoid cells (ILC3). Mice lacking RORγt are devoid of lymph nodes and Peyer's patches due to the lack of Lymphoid tissue inducer cells (LTi), a subpopulation of ILC3s and important drivers of lymphoid organogenesis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.04	1.61	1.23	0.84	0.53	0.35	0.23	0.08
Corrected OD	1.96	1.53	1.15	0.76	0.45	0.27	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse RORγt spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	97
Cell Lysates(n=8)	92-106	102

 

| Linearity

Samples were spiked with high concentrations of Mouse RORγt and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98